Teacher蔡: (1) Fig 1C western 太髒, not qualified for publication (要改進!). (2) EAD clone 88 is a good one for IFA (3) Akata strain 比 B95-8 transforms B cell 能力弱很多. (3) Dox-treated ERKV中 knock-downKR (siRNA),用以證實ER是透過KR做事... (4) ACIF是做好EBNA1 IFA之唯一良藥. (5)淑君的Blood paper中述及primary infection可有Z, R, 一直到VCA. (6)葉醫師的differentiation系統為Sphenoidal sinus, 並未有un-differentiated cells.
Teacher陳M.-R.: (1) MA 220 is a good antibody for Dox-EREV8 late gene expression (又是Fig 1C!) (2) Mechanism of ER-mediated activation of KR promoter.
Teacher陳J.-Y.: 要做與疾病,癌症有關的為佳!
SFL: (1) 一篇會令人開悟的文章, 這裡.(2) 293的Vitronectin receptor是怪胎 (ITGAVB1). (3)NA中ER的量與EREV8中ER的量是可以比較估算的.
Jan 28, 2010
Jan 22, 2010
B292 相關
1/21/2010 (Thu) 第一次討論會議
Speaker 1-北醫劉景平: Structures of 292, J-30, SAHA etc. One OCH3 group in 292 is enough, two OCH3 increases IC50 from 50 nM to 1400 nM; replace with OH increases IC50 from 50 nM to 500 nM. SAHA is a planer structure, quite stable. SAHA is a pan-HDACi, with uM range. B291 is also a pan-HDACi, with 100 nM range. Wishing 292 is more specific.
Speaker 2-程韻靜: Effects of 292 (1/3) inhibits tubulin polymerization in KB cell; (2/3) inhibits tube formation in SH-SY5Y; (3/3) Animal models (CAM, xenograft in H460 58.7% inhibition, orthotopic PANC-1 with Luc expression); Calcium influx was increased in Taxol or J-30 (not as good as Taxol) treated neuronal cells, but neither SAHA nor 292 had effects in calcium influx; While most cells exhibit 50~100 nM IC50, HUVEC is 42 uM and WI-38 is 12 uM (so interesting!!!)
Speaker 3-夏興國: (1) B292 and EMT seemed not have correlation in breast and lung cancer cells (2) B292 and Adhesion: CL1-0 扁平+292 (uM) =扁平. CL1-5 細長+292 (nM)=morphological changes and tubulin disperse.... (但butyrate, TSA, SAHA都能造成相同現象(FAK and paxillin increases) 耶...
Speaker 4-吳仲峻: TWO1 (72 nM), NA (35 nM with EAD 5~10%), HONE-1 (66 nM), HA (42 nM).
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11/1/09 SFL wrote:
與PrCA/AR有關:Taken together, our studies suggest that HDAC6 regulates AR hypersensitivity and nuclear localization, mainly via modulating HSP90 acetylation.
與Tau有關:To determine the relationship of tau protein and increased tubulin acetylation, we studied the effect of tau on the acetylation-deacetylation of tubulin. Our results indicate that tau binds to the tubulin-deacetylase, histone deacetylase 6 (HDAC6), decreasing its activity with a consequent increase in tubulin acetylation.
有趣, 有趣..
有空要瞭解一下HSP90,就這麼查吧. "hsp90 review AND cell cycle [jour]"
SAHA 相關 Chiang's recent paper [Here].
Speaker 1-北醫劉景平: Structures of 292, J-30, SAHA etc. One OCH3 group in 292 is enough, two OCH3 increases IC50 from 50 nM to 1400 nM; replace with OH increases IC50 from 50 nM to 500 nM. SAHA is a planer structure, quite stable. SAHA is a pan-HDACi, with uM range. B291 is also a pan-HDACi, with 100 nM range. Wishing 292 is more specific.
Speaker 2-程韻靜: Effects of 292 (1/3) inhibits tubulin polymerization in KB cell; (2/3) inhibits tube formation in SH-SY5Y; (3/3) Animal models (CAM, xenograft in H460 58.7% inhibition, orthotopic PANC-1 with Luc expression); Calcium influx was increased in Taxol or J-30 (not as good as Taxol) treated neuronal cells, but neither SAHA nor 292 had effects in calcium influx; While most cells exhibit 50~100 nM IC50, HUVEC is 42 uM and WI-38 is 12 uM (so interesting!!!)
Speaker 3-夏興國: (1) B292 and EMT seemed not have correlation in breast and lung cancer cells (2) B292 and Adhesion: CL1-0 扁平+292 (uM) =扁平. CL1-5 細長+292 (nM)=morphological changes and tubulin disperse.... (但butyrate, TSA, SAHA都能造成相同現象(FAK and paxillin increases) 耶...
Speaker 4-吳仲峻: TWO1 (72 nM), NA (35 nM with EAD 5~10%), HONE-1 (66 nM), HA (42 nM).
------------------------------------------------------------------------------
11/1/09 SFL wrote:
與PrCA/AR有關:Taken together, our studies suggest that HDAC6 regulates AR hypersensitivity and nuclear localization, mainly via modulating HSP90 acetylation.
與Tau有關:To determine the relationship of tau protein and increased tubulin acetylation, we studied the effect of tau on the acetylation-deacetylation of tubulin. Our results indicate that tau binds to the tubulin-deacetylase, histone deacetylase 6 (HDAC6), decreasing its activity with a consequent increase in tubulin acetylation.
有趣, 有趣..
有空要瞭解一下HSP90,就這麼查吧. "hsp90 review AND cell cycle [jour]"
SAHA 相關 Chiang's recent paper [Here].
Jan 16, 2010
Jan 7, 2010
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