OSCC Cell Line STR information 20150312 更新版
由 sufang 在 一, 03/30/2015 - 08:45 發表 Oral Cancer OSCC Cell Line STR analysis
Dear all,
更新oral cancer相關細胞株之STR資訊
共有16個細胞株, 其中DOK, FaDu, HOK, HSC-3, KB, OEC-M1, SCC-15, SCC-9, Tu-183(共9株)有送件STR的資訊(詳如附件)
須請大家注意的資訊如下:
1. HSC-3, 於Dr.郭靜娟's lab的新HSC-3為真, 請非此來源者把舊的細胞扔了吧! (p.s Dr. 李岳倫於2014年7月及9月分別定的STR結果同新HSC-3, 推測亦為可用細胞株)
2. KB, 於Dr.郭靜娟's lab定STR結果為HeLa細胞, ATCC/JCRB資料庫也證明KB為HeLa. 請勿使用此細胞株!
3. SCC-15, Dr.李岳倫's lab 於2014年9月及10月分別定的STR結果皆與SAS細胞株相符(90.9% match), 使用此細胞株請小心!
4. Tu-183, 於Dr.江士昇's lab於2014年12月所定STR結果未比對到現今資料庫中任何細胞株, 推測可能為可用細胞株. 但Dr. 江提供資訊:expression pattern與預期不同, 大家使用此細胞株請小心!
以上,
如有問題或錯誤的地方, 敬請不吝指正! 謝謝!
敬祝 研究順利
嬿如 敬上
原檔按我下載 (2015_0320_cell_lines_str_information20150312ding_gao.xlsx)
Mar 29, 2015
2015-03-10 Journal Club Paper
各位老師、學長學姊大家好:
附檔是我這周要報告的paper
請查收 謝謝大家
NRF2-driven miR-125B1 and miR-29B1 transcriptional regulation controls a novel anti-apoptotic miRNA regulatory network for AML survival
附檔是我這周要報告的paper
請查收 謝謝大家
祝 研安
韻涵 敬上
韻涵 敬上
Mar 13, 2015
2015/01/30 國長會議
由 sufang 在 五, 03/13/2015 - 08:29 發表 Pre-published 國長會議 長庚
國衛院長庚雙邊會議 2015/01/30 (五) at 林口長庚
王陸海: miR491-3p is responsible for oral cancer metastasis
葉大森: bile duct cancer (miRNA 200下降), liver cancer (miRNA 200 上升，which is correlated with EMT)
陳鈴津教授介紹 閻紫宸教授另外一個專長是 radio pharmatheutical research (新藥開發 GMP等等)
-----------------------
趙盈凱 副教授【胸腔外科】可吸收性生物醫材在食道癌之應用
東方人食道癌 是由ABC引起的、西方的食道癌則多由胃食道逆流
證實CCRT+Surgert is better therapy than just surgery
-Big Database (2400 病人 由該醫師和助理慢慢build up)
-健保加值資料庫
-cisplatin membrane
biodegradable polymers in thoracic disease
-----------------
陳立宗 特聘研究員兼所長 【國衛院癌症研究所】 Pancreatobiliary Cancer Clinical Trial in Taiwan
–TCOG很多東西需要長庚醫院幫忙 e.g. 陳敏夫教授 adjuvant interferon alpha 2b TCPG T1297 (this is a negative study, indicating that Taiwanese surgery was very successful)
–原來文獻建議 肝癌患者 開完刀後若能 加上抗病毒藥物治療 可有效降低recurrence rate.
但是由TCOG的結果看來, not necessary.
-----------------
葉大森: bile duct cancer (miRNA 200下降), liver cancer (miRNA 200 上升，which is correlated with EMT)
陳鈴津教授介紹 閻紫宸教授另外一個專長是 radio pharmatheutical research (新藥開發 GMP等等)
-----------------------
趙盈凱 副教授【胸腔外科】可吸收性生物醫材在食道癌之應用
東方人食道癌 是由ABC引起的、西方的食道癌則多由胃食道逆流
證實CCRT+Surgert is better therapy than just surgery
-Big Database (2400 病人 由該醫師和助理慢慢build up)
-健保加值資料庫
-cisplatin membrane
biodegradable polymers in thoracic disease
-----------------
陳立宗 特聘研究員兼所長 【國衛院癌症研究所】 Pancreatobiliary Cancer Clinical Trial in Taiwan
–TCOG很多東西需要長庚醫院幫忙 e.g. 陳敏夫教授 adjuvant interferon alpha 2b TCPG T1297 (this is a negative study, indicating that Taiwanese surgery was very successful)
–原來文獻建議 肝癌患者 開完刀後若能 加上抗病毒藥物治療 可有效降低recurrence rate.
但是由TCOG的結果看來, not necessary.
-----------------
Mar 6, 2015
ds-cDNA prep for gene fusion detection
3/6/2015 DR 提醒
1: Daw-Yang seems to use Clontech ds-cDNA prep for single cell Seq + Tagmentation
2: There is no way to add specific primer in this way...
According to Daw-Yang: I will use Nexera XT for DNA prep.
Figure 1 AMP for targeted RNA and DNA sequencing (pdf 4231).
Double-stranded cDNA (dscDNA) synthesis starts with total nucleic acid or RNA from fresh or FFPE material without ribosomal RNA or genomic DNA(gDNA) depletion. Solid-phase reversible immobilization (SPRI)-cleaned double-stranded cDNA or fragmented genomic DNA is processed with end repair and dA tailing, directly followed by ligation with a half-functional adapter. SPRI-cleaned, ligated fragments are amplified with 10–14 cycles of multiplex PCR1 using gene-specific primers (GSP1 pool) containing a PCR multiplexing tag (brown) and a primer complementary to a portion of the universal ligated adapter (short green). An unknown fusion partner or target sequence is indicated by a question mark. SPRI-cleaned PCR1 amplicons are amplified with a second round of 10-cycle multiplex PCR2 using a combination of GSP2 pool nested gene-specific primers (3' downstream of GSP1), which are tagged with the second adapter sequence specific for Ion Torrent (black-blue) or Illumina (black-red, subsequently tagged with an indexing primer (red-orange-purple)), and a second nested primer against the ligated universal adapter (long green). After a final SPRI cleanup, the target amplicon library is ready for quantitation, downstream clonal amplification and sequencing.
Fluidigm 4-Primer Amplicon Tagging
The Access Array 4-primer amplicon tagging scheme is based on a step-out four primer PCR strategy where tagged target-specific (TS) primer pairs are combined with sample-specific primer pairs that contain a barcoding sequence and the adaptor sequences used by Illumina sequencing systems. By incorporating sample-specific barcodes, all 2,304 PCR products generated from a 48.48 Access Array Integrated Fluidic Circuit (IFC) are unique and can be pooled together to run in a single sequencing experiment.
Nextera_xt_sample_prep
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