清大生科院 Biotech Job Fair

外來文轉發

敬邀參加 5/29/2014 清大生科院 Biotech Job Fair

各位生技產業及教育界先進您好:

清大生命科學院長期培育跨領域的優秀生物科技、生物醫學人才，包括學士、碩士、博士、及博士後研究員。

清大生命科學院生科院職涯發展中心(NTHU College of Science Career Development Center)將於五月二十九日(星期四)下午舉辦『生物科技明日之星校園徵才(2014 NTHU Biotech Job Fair)』，敬邀 貴單位提供半年內預計徵聘人才的企業界職缺資訊。

敬請於四月十四日(星期一)前提供「企業人才需求表」，我們會依據 貴單位的人才需求內容收集本院及本校相關領域人才資訊，並積極媒合雙向互動。

歡迎您於五月二十九日蒞臨本院參觀及指教。

敬祝    順心如意

國立清華大學生命科學院職涯發展中心

黎耀基、張大慈敬邀  

本活動聯絡人：楊子儀小姐 E-mail：show@life.nthu.edu.tw 電話：03-5742165

Primary NP cell from CELPROGEN

由 ingrid 在 四, 05/22/2014 - 15:53 發表 EBV and KSHV Cell Lines EBV infection Ingrid

瑞柏代理

網頁 http://www.celprogen.com/index.php?route=product/product&filter_name=nasophary&product_id=2028&path=26_44_45_47 

Celprogen原廠配合的廠商為World Courier。

原廠並無販售coating matrix,所以可能還是建議需要購買它們家的耗材。

20140527 Journal club paper

Dear all:
          下週二輪到我報告了，paper請按我下載。
   paper title:The E3 ligase Cbl-b and TAM receptors regulate cancer metastasis via natural killer cells

                                                                                                                           Can

很玄但是非常重要的文章 CTCF

由 sufang 在 四, 05/15/2014 - 15:44 發表 EBV and KSHV chromosome conformation capture (3C) cohesion CTCF Topologically associated domain (TAD)

Cell. 2014 May 8;157(4):950-63. doi: 10.1016/j.cell.2014.03.025.

 

Predictive polymer modeling reveals coupled fluctuations in chromosome conformation and transcription.

Giorgetti L1, Galupa R1, Nora EP2, Piolot T1, Lam F1, Dekker J3, Tiana G4, Heard E5.
Author information

    1Institut Curie, 26 Rue d'Ulm, 75248 Paris Cedex 05, France; CNRS UMR3215, 75248 Paris Cedex 05, France; INSERM U934, 75248 Paris Cedex 05, France.
    2Institut Curie, 26 Rue d'Ulm, 75248 Paris Cedex 05, France.
    3Program in Systems Biology, Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 368 Plantation Street, Worcester, MA 01605-0103, USA.
    4Dipartimento di Fisica, Università degli Studi di Milano and INFN, Via Celoria 16, 20133 Milano, Italy. Electronic address: guido.tiana@unimi.it.
    5Institut Curie, 26 Rue d'Ulm, 75248 Paris Cedex 05, France; CNRS UMR3215, 75248 Paris Cedex 05, France; INSERM U934, 75248 Paris Cedex 05, France; Collège de France, 11 place Marcelin-Berthelot, Paris 75005, France. Electronic address: edith.heard@curie.fr.

Abstract

A new level of chromosome organization, topologically associating domains (TADs), was recently uncovered by chromosome conformation capture (3C) techniques. To explore TAD structure and function, we developed a polymer model that can extract the full repertoire of chromatin conformations within TADs from population-based 3C data. This model predicts actual physical distances and to what extent chromosomal contacts vary between cells. It also identifies interactions within single TADs that stabilize boundaries between TADs and allows us to identify and genetically validate key structural elements within TADs. Combining the model's predictions with high-resolution DNA FISH and quantitative RNA FISH for TADs within the X-inactivation center (Xic), we dissect the relationship between transcription and spatial proximity to cis-regulatory elements. We demonstrate that contacts between potential regulatory elements occur in the context of fluctuating structures rather than stable loops and propose that such fluctuations may contribute to asymmetric expression in the Xic during X inactivation. Copyright © 2014 Elsevier Inc. All rights reserved. PMID: 24813616

好康相報

清華大學生命科學院職涯發展中心(NTHU College of Science Career Development Center)將於五月二十九日(星期四)下午舉辦"生物科技明日之星校園徵才" (2014 NTHU Biotech Job Fair)，目前已有19家廠商提供62項職缺資訊（博士職缺約需14名、碩士職缺約需40名以上及學士職缺約需14名），請點閱〝job fair(公告)〞或活動網址http://2014JobFair.life.nthu.edu.tw，（職缺訊息不定時更新）。

歡迎各位老師轉知活動訊息給實驗室應屆畢業生，敬請於五月二十日(星期二)前提供"履歷表"(點選下載)，E-mail寄給活動聯人-楊子儀小姐收show@life.nthu.edu.tw，我們會依據各位的意向資料提供給廠商進行媒合作業。

國立清華大學生命科學院職涯發展中心

黎耀基、張大慈、焦傳金 敬邀

DOK_MOLAS analysis related

由 sufang 在 三, 05/07/2014 - 16:40 發表 Oral Cancer DOK Molas Oral Cancer

提醒:

MOLAS入口:

• 40T/N: 請用「Library comparison」, compare "average" and "N".
• 4NQO mouse: 可按「Cludtering」--> view clustering result 玩玩看。
• DOK project.

login:

pwd:

05/07 SFL小notes:

• ASS1 in NNK and AC treatment 都有下降。
• Shiah's AC vs DOK3: "GO: microtubule binding" ranks the best p-score. 共有17個genes invovled (ex kinesin families), 多是表現上升趨勢。
• 娟' AC vs DOK2: "GO:mitotic cell cycle" ranks the best. 而且involve的基因 member高達138個, AURKA, AURKB都在裡面耶!! (讀值都大於500, 倍數均增加兩倍以上)

2014-05-13 Journal Club Paper

Dear all:  如題~ 謝謝大家 (論文按我下載)

Title: Regulation of adipose oestrogen output by mechanical stress

綜合此文下方二個回應

DOK related

註二: 還有靜娟的10片array和夏博的5片可能不要放在一起比: 在"library comparison"我用DOK1/DOK2當pool A, DOK3當pool B, 或是比AC50與AC50_1, NNK6與NNK6_1, 都有許多基因大於2倍以上。(結果檔案)

         註二之ㄧ: DOK1 vs DOK2 >=二倍差異有2069個基因 (有這麼多! unexpected)。

         註二之二: DOK1 vs DOK3 >=二倍差異有2162個基因。

         註二之三: DOK2 vs DOK3 >=二倍差異有3192個基因。

         註二之四: pool (DOK1, 2) vs DOK3 >=二倍差異有2065個基因。

         註二之五: AC50 vs AC50_1 >=二倍差異有4931個基因。

         註二之六: NNK6 vs NNK6_1 >=二倍差異有2818個基因。

         註二之七: DOK3 vsAC50_short >=二倍差異有1473個基因。

         註二之八: DOK3 vs NNK_short >=二倍差異有4980個基因。

註三: 上次meeting時靜娟提到的幾個NRF2/ARE target, 先睹為快。​​(註:這是用"Enrichment Analysis"把基因name輸進去做出來的)

註四: DOK1/DOK2 vs NIC4, 5, 6。(結果檔案)

註五: DOK1/DOK2 vs NNK4, 5, 6 。(結果檔案)

註六: DOK/DOK2 vs AC50, AC100。(結果檔案)

註七: DOK3 vs AC_short。(結果檔案)

​註八: DOK3 vs NNK_short。(結果檔案)

第十九屆台灣癌症聯合學術年會 (ACOS-TJCC, 2014/5/2–4)

大會網址由此進~

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Day 1, May 2 (Friday)

下午長庚閻紫宸 OI-03 MR/PET in Oncology Imaging
INTEGRATING IMAGING DATA WITH INFORMATION FROM GENOMICS IN ONCOLOGY
Tzu-Chen Yen  Molecular Imaging Center, Chang Gung Memorial Hospital and University, Taiwan
        As a physician and researcher in medical imaging, we have been fascinated by the rapid and dramatic improvement of imaging capabilities over the past decade. Meanwhile, we have also witnessed an explosive development of the 'omics' technologies - genomics, proteomics, metabolomics, etc. over the years. Now that we are living in the “-omics” era, it has become an emergent need to integrate imaging data with the “-omics” data. More importantly, role of imaging should be defined in such integration by identifying its advantages. With such integration, we see a person truly as whole - macroscopically and microscopically. However, such integration is challenging. A few groups have been working on this emergent topic so far, but not too many yet. We initiated our work since 2007 with the start-up project of head and neck cancers for at least two reasons. First, head and neck cancers are important cancers in Taiwan because it is the top ranking cancer in male between 30-60 years old in Taiwan. Second, the treatment regimens for head and neck cancers in our hospital will follow a fixed protocol and most of them will have a radical surgery. Therefore, we may have a chance to study the “-omics” from their bio- and tissue specimens. We hope that through this start-up study, we can achieve a few goals: (1) Promoting the importance of imaging/omics data integration; (2) Letting people know this is feasible as well as what are the state of the arts; (3) Facilitating the interdisciplinary collaboration between different groups for pooling data together and (4) Stimulating the technological developments for such endeavors. Herein, I wish to share with you some of our preliminary data.