Summary for the discussion

Studying plans:

1. How is DDR1 regulated by miRNA?

   -- We will discuss the detail.

2. How many signaling pathways are involved in oral tumorigenesis?

   How many signaling pathways are involved in oral premalignant lesions?

   -- There are 12 important signaling pathways invovled in tumorigenesis.

3. Is EGCG working on 12 signaling pathways?

   Is EGCG or EGCG/curcumin a good chemoprevention agent in premalignant lesions?

0215Lab meeting-蔡小丸

1. 完成三組tissue microarray的DDR1, collagen type 1及collagen type 4的染色, 初步計分結果, 似乎DDR1的大量表達與collagen type1的大量表達較為相關,而DDR1大量表達也與疾病的grade較相關
2. 比較OSCC cells DDR1 tyrosine phosphorylation的情形, 結果為DOK, C9, OC-3, OEC-M1, TW2.6>HSC-3, SCC-15, T47D>HEK293, 發現DDR1 tyrosine phosphorylation的程度與gilvec對於這些細胞的抑制能力,沒有很好的相關性, 然而發現在OEC-M1, TW2.6, HSC-3, SCC-15這幾組IP DDR1 sample中, 發現約略80-85kDa的DDR1-associated protein有強烈的tyrosine phosphorylation, 但此蛋白為何未知
3.此外 DDR1的活性反映出的下游為何未知 (但排除非p-MAPK及p-AKT)
4.利用Q-PCR分析一系列oral cells的DDR1 isoforms的表達, 結果發現大多細胞只表達1a及1b這兩種isoforms, 而mRNA表達量也與蛋白表達量具相關性

2/13/2012 OrCA Meeting

1. 下次開會: 6月
2. CA OrCA PIs + 王副院長、莊JL、陳YR、黃HG (RA:雪君)、江主秘。
3. BioSignature Program: Leader/Chang Gung 核醫科主任閻紫宸, looking for cause-effect marker. The third meeting will be held on March-8; focus on miRNA/epigenetics/DNAs; funding source, Academia Sinica.
4. 王副院長Lab學生報告 (Wei-Chieh Huang)—長庚閻醫師 has 53 OrCA T/N pairs; miR-491-5p is involved in cell migration and invasiveness, targeting GIT1 and RAB1; OC-3 and C9 were used。
5. Dr. 莊JL
Cellular prion is increased by alcohol consumption involved in tumorigenesis of OSCC
PrpSC/PRNP is significantly up-regulated in OSCC
以電腦判讀TMA result
Second primary tumor (what is the definition of second primary? second primary vs. recurrent)
Alcohol induced TGF-b1 elevaltion is modulated by PrpC
6. 雪君:
Small heat shock protein
HSP27, 60, 70, 90, 110 MW 10–30 are called as small HSP
HSPB1=HSP27
She uses HOK, Cai-27, OC3, OEC-M1, Ca9-22 as cell models
HSP27/HSPB1 enhances OSCC apoptosis via repression in response to oxidative stress
H2O2 (50 uM), ANE (10 ug/ml), ANE (30 ug/ml)
PKCdelta and PTP1B function as downstream of HSP27/HSPB1 in response to oxidative stress.

2012/2/1 Lab Meeting- 小夏

1. 計數TW05TetLuc/ER dox treat 1, 2, 3, 5, 6, 8天的細胞數。
2. 以467確認Rta expression pattern 在TW01TetER及TW05TetER不同。 Rta expression level by WB: #19 ≧#16＞TW05TetER。 將再確認p21, c-Myc, p53......的表現。
3. 準備HEK293, DOK, HSC3, SCC15, OECM1的genomic DNA來確認DDR1的mutation位點。
4.準備HEK293, DOK, HSC3, SCC15, OECM1的cDNA來確認DDR1的isoform。