Dear All:
我今天要報告的paper是
The tumorigenic FGFR3-TACC3 gene fusion escapes miR-99a regulation in glioblastoma. 全文請按我下載，謝謝！
Mar 12, 2013
Mar 4, 2013
02/27 Lab Meeting - Ingrid
1. 比較Imatinib、Dasatinib及Nilotinib對於OSCC cells生長的影響。
2.進行PD173074(FGFR inhibitor)對於OSCC cells生長的影響。發現具有fusion gene的C9細胞的確有受到抑制生長。
3.分享關於DNA定序的演進。
2.進行PD173074(FGFR inhibitor)對於OSCC cells生長的影響。發現具有fusion gene的C9細胞的確有受到抑制生長。
3.分享關於DNA定序的演進。
Feb 27, 2013
補遺：mir-17-92 cluster
mir-17-92 cluster是由MIR17HG這個gene transcribed出來的：
MIR17HG: This gene is the host gene for the MIR17-92 cluster, a group of at least six microRNAs (miRNAs) that may be involved in cell survival, proliferation, differentiation, and angiogenesis. Amplification of this gene has been found in several lymphomas and solid tumors. Two non-protein coding transcript variants have been found for this host gene, but only the longest is a polycistronic transcript containing the MIR17-92 cluster.
UCSC連結
MIR17HG全長為6756bp,裡面的六各microRNA precusors位置從
mir-17 (chr13:92,002,859)
mir-18a (chr13:92,003,005)
mir-19a (chr13:92,003,145)
mir-20a (chr13:92,003,319)
mir-19b-1 (chr13:92,003,446)
mir-92a-1 (chr13:92,003,568)
UCSC連結
彼此靠得非常近，總結來說，他們來自a single primary transcript，受到相同的調控，沒有獨自的promoter。
MIR17HG: This gene is the host gene for the MIR17-92 cluster, a group of at least six microRNAs (miRNAs) that may be involved in cell survival, proliferation, differentiation, and angiogenesis. Amplification of this gene has been found in several lymphomas and solid tumors. Two non-protein coding transcript variants have been found for this host gene, but only the longest is a polycistronic transcript containing the MIR17-92 cluster.
UCSC連結
MIR17HG全長為6756bp,裡面的六各microRNA precusors位置從
mir-17 (chr13:92,002,859)
mir-18a (chr13:92,003,005)
mir-19a (chr13:92,003,145)
mir-20a (chr13:92,003,319)
mir-19b-1 (chr13:92,003,446)
mir-92a-1 (chr13:92,003,568)
UCSC連結
彼此靠得非常近，總結來說，他們來自a single primary transcript，受到相同的調控，沒有獨自的promoter。
Feb 19, 2013
Feb 6, 2013
Jan 29, 2013
2013/02/05 Journal Club
大家好:
小龍年前最後一次journal club 要跟大家分享的paper 請下載連結檔案。Title: Fibroblast Growth Factor Receptor 3 (FGFR3) Is a Strong Heat Shock Protein 90 (Hsp90) Client
小龍年前最後一次journal club 要跟大家分享的paper 請下載連結檔案。Title: Fibroblast Growth Factor Receptor 3 (FGFR3) Is a Strong Heat Shock Protein 90 (Hsp90) Client
Jan 21, 2013
20130122 Journal Club Paper
大家好，
連結檔案是我這週要報告的paper，請查收，謝謝！
Title: Activation of TGF-b Pathway by Areca Nut Constituents: A Possible Cause of Oral Submucous Fibrosi.
連結檔案是我這週要報告的paper，請查收，謝謝！
Title: Activation of TGF-b Pathway by Areca Nut Constituents: A Possible Cause of Oral Submucous Fibrosi.
Jan 17, 2013
20130116Lab meeting-小丸
1. 利用pooled shDDR1 clones探討DDR1對於OSCC cells的影響，結果發現抑制DDR1表達造成OSCC cells數目降低及細胞凋亡數目增加，而p-SHC、p-SHP2、p-MAPK、CDK1及bcl2為參與DDR1 pro-survival 路徑的重要分子。
2. 比較一系列DDR1 inhibitors (Imatinib、Dasatinib及Nilotinib )對於OSCC cells生長的影響，其中Dasatinib對於OSCC cells的生長抑制效果顯著，但其為廣效型抑制劑，相較之下對DDR1專一性較差，因此，之後實驗仍會著重於Imatinib及Nilotinib當作DDR1抑制劑，觀察對於OSCC cells生長的影響。
3. 利用HOK探討collagens是否會增加DDR1及collagens的protein及mRNA level表達，結果發現collagen typeIV扮演重要角色。
2. 比較一系列DDR1 inhibitors (Imatinib、Dasatinib及Nilotinib )對於OSCC cells生長的影響，其中Dasatinib對於OSCC cells的生長抑制效果顯著，但其為廣效型抑制劑，相較之下對DDR1專一性較差，因此，之後實驗仍會著重於Imatinib及Nilotinib當作DDR1抑制劑，觀察對於OSCC cells生長的影響。
3. 利用HOK探討collagens是否會增加DDR1及collagens的protein及mRNA level表達，結果發現collagen typeIV扮演重要角色。
Jan 10, 2013
Jan 5, 2013
DDR1, Integrin, Cell adhesion and more...
Discoidin Domain Receptors Promote α1β1- and α2β1-Integrin Mediated Cell Adhesion to Collagen by Enhancing Integrin Activation. (PLoS One. 2012;7(12):e52209)
Xu H, Bihan D, Chang F, Huang PH, Farndale RW, Leitinger B.
The discoidin domain receptors, DDR1 and DDR2, are receptor tyrosine kinases that bind to and are activated by collagens. Similar to collagen-binding β1 integrins, the DDRs bind to specific motifs within the collagen triple helix. However, these two types of collagen receptors recognize distinct collagen sequences...
Xu H, Bihan D, Chang F, Huang PH, Farndale RW, Leitinger B.
The discoidin domain receptors, DDR1 and DDR2, are receptor tyrosine kinases that bind to and are activated by collagens. Similar to collagen-binding β1 integrins, the DDRs bind to specific motifs within the collagen triple helix. However, these two types of collagen receptors recognize distinct collagen sequences...
Jan 2, 2013
20130102 Journal Club Paper
Dear all,
Attached is (link me here) the paper I will present next Tuesday, please received. W-H Lee
Title: AMPK Promotes p53 Acetylation via Phosphorylation and Inactivation of SIRT1 in Liver Cancer Cells
Title: AMPK Promotes p53 Acetylation via Phosphorylation and Inactivation of SIRT1 in Liver Cancer Cells
Dec 19, 2012
專家的話
又看到一篇有趣的文章了，米那桑!
Lab Life: Balancing act.
快快讀過的心得是：
Lab Life: Balancing act.
快快讀過的心得是：
- 利用下載paper的記錄作為一個scientist花在研究相關工作上的指標，發現美國人工作得較晚、中國人則多利用週末兩天來做研究。
- 文中提及每週花60(中國)~80(美國)小時在研究工作上是吃這行飯人的宿命。不過，大多數花長時間工作做科研的人卻是樂在其中。
- 問題是投資報酬率，就是說creativity/productivity卻不是和長時間工作成正比。
- New idea常常來自較放鬆愉快的情境中，因此要常常注意自己的狀態是不是太緊繃，也要發掘一、二樣可以讓自己心境reset的嗜好，並且適度take a break.
- 要懂得規劃事情的優先順序，對那些輕而急的事情要堅持做完，對可以緩一點再做的事情要懂得跟太demanding的主管說no.
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