2021/05/14 Seminar 暫停公告
因受到新冠肺炎疫情影響，謹依所長之囑，公告周知。
本所Seminar自5月17日起至6月底將暫停舉行，謝謝!
May 17, 2021
Feb 8, 2021
p-EMT 相關文獻
2018_02_Single cell RNA-seq highlights a role for a partial EMT in head and neck cancer (PubMed Link)
2019_12_Immunohistochemical quantification of partial-EMT in oral cavity squamous cell carcinoma primary tumors is associated with nodal metastasis (PubMed Link)
2020_11_Molecular margins in head and neck cancer- Current techniques and future directions (PubMed Link)
______________YHL Research Day Abstract Final______________
(Background) Partial epithelial-to-mesenchymal transition (p-EMT) is a molecularly pathologic feature revealed by single-cell RNA-seq and immunohistochemistry (IHC) studies of oral squamous cell carcinoma (OSCC) tissues comprising both primary and metastatic tumors. Compared with conventional EMT, tumor cells with the p-EMT program retain genes of epithelial origin (e.g. TP63, SPRR1B); express only partial mesenchymal (e.g. SNAI2) and extracellular matrix genes confined to the invasion front (e.g. PDPN, LAMB3, LAMC2). Quantitative IHC studies further demonstrated that p-EMT is an independent prognostic signature for nodal metastasis, which may aid in decision-making for an N0 neck dissection and/or adjuvant modalities.
(Aims) OC3 and TW2.6 are Taiwanese OSCC cell lines expressing distinct sets of EMT marker genes in cell culture, reminiscent of the EMT and p-EMT programs, respectively. In addition, we showed that TW2.6-derived xenograft tissues displayed prominent angiolymphatic- and perineural invasions that involve DDR1 kinase activity and CDH1 protein stability at the cell-cell junctions [Chen YL et al., Cancers, PMID: 32244515]. Here, by combining bulk RNA-seq analysis and IHC assay of OC3 and TW2.6-derived xenograft tissues, we aim to (1) validate genes and pathways attributable to the TW2.6 p-EMT program; (2) identify stromal cell subsets enriched in the TW2.6-CDX tissues; (3) associate p-EMT OSCC tissues with clinical outcomes.
(Results) As a whole, computational analyses using R limma, GSEA and DAVID consistently revealed that genes and cellular pathways related to the angiogenesis and immune processes are statistically enriched in the stroma of TW2.6-CDXs, compared to that of the OC3-group. In addition, CIBERSORTx and GSEA using custom gene matrix transposed files predicted that the two subsets of cancer-associated fibroblasts (CAFs), i.e. myofibroblastic (myCAF) and inflammatory (iCAF), are enriched in the OC3- and TW-2.6 stroma, respectively. To validate the bioinformatic data, IHC assays using Pecam1 (Cd31) and Adgre1 (F4/80) antibodies were conducted in each xenograft tissue. IHC results confirmed that (1) the microvascular densities of the TW2.6-CDX tissues are statistically higher than that of the OC3 group; (2) the major immune subsets in both OC3- and TW2.6 CDXs are macrophages. Interestingly, integrated correlational analyses of RNA-seq data suggested that certain immune related genes denoted in the CIBERSORTx might indeed originate from the iCAFs, including the expression of Cxcl1, Cxcl5, Cxcl9, Cxcl10, and Cxcl12. The clinical outcomes associated with p-EMT program of oral cancer specimens are under progress.
Feb 7, 2021
CD47, Mina Bissel, ECM, teratoma, Hybrid E/M signatures
2017_04_A CD47-associated super-enhancer links pro-inflammatory signaling to CD47 upregulation in breast cancer (pdf 5258) [IL Weissman Lab]
2020_02_Comparative study of Transcriptomics-based scoring metrics for the epithelial-hybrid-mesenchymal sepctrum (psd 5609) [Chakraborty ... Jolly]
Cancer cell stemness (for TYL)
2018_07_Targeting breast cancer stem cell state equilibrium through modulation of redox signaling [Cell Metabolism Lo et al.] 雲端文獻下載連結
2018_11_Pluripotency transcription factors and metabolic reprogramming of mitochondria in tumor-initiating stem-like cells [Antioxidants & Redox Signaling Keigo Machida] 雲端文獻下載連結
2020_09_Oxidative metabolism drives immortalization of neural stem cells during tumorigenesis [Cell Bonnay et al.] 雲端文獻下載連結(本文)
雲端文獻下載連結(介紹文) A metabolic bottleneck for stem cell transformation [Baksh and Fuchs]
Feb 5, 2021
2020-12-13 亞太生醫矽谷精準醫療旗艦計畫成果展示暨學術硏討會
Dec 30, 2020
International Conference on Recent Advances in Precision Medicine and Population Health
Dr. Huey-Kang Sytwu
Opening Remarks
Vice President, National Health Research Institutes, Taiwan
Dr. Hou-Chuan Chen
Opening Remarks
Deputy Superintendent, Taoyuan General Hospital, Ministry of Health and Welfare, Taiwan
Dr. Kung-Yee Liang
Special Lecture
President, National Health Research Institutes, Taiwan
Dr. Yik-Ying Teo
Opportunities and Challenges in the Precision Public Health of Non-Communicable Diseases
Dean, NUS Saw Swee Hock School of Public Health, SINGAPORE
Dr. Feza Korkusuz
Joint Cartilage Regeneration
Professor, Department of Sports Medicine, Hacettepe University, TURKEY
Dr. Shih-Feng Tsai
Big Data Analysis and Application for Precision Medicine
Distinguished Investigator, Institute of Molecular and Genomic Medicine, National Health Research Institutes, Taiwan
Dr. Feng-Huei Lin
Bioengineering in Precision Medicine
Distinguished Investigator and Director, Institute of Biomedical Engineering and Nanomedicine National Health Research Institutes, Taiwan
Dr. Shu-Hsing Cheng
A multicenter, Randomized, Open-label, Controlled Trial to Evaluate the Efficacy and Tolerability of Hydroxychloroquine in Adult Patients with Mild to Moderate Coronavirus Disease 2019 (COVID-19)
Attending Physician and Director, Department of Education and Research, Taoyuan General Hospital, Ministry of Health and Welfare, Taiwan
Dr. Chun-Yuan Lin
Aim to Next Generation Psychiatry
Attending Physician and Director, Department of General Psychiatry, Tsaotun Psychiatric Center, Ministry of Health and Welfare, Taiwan
Dr. Shiu-Feng Huang
Introduction of NHRI Biobank
Investigator and attending physician, Institute of Molecular and Genomic Medicine, National Health Research Institutes
Dr. Chien-Yu Cheng
Clinical Sample Fast-screening Evaluation and Examination for COVID-19
Attending Physician and Director, Department of Infectious Diseases, Taoyuan General Hospital, Ministry of Health and Welfare, Taiwan
Dr. Hsiu-Hsueh Tseng
Rapid Execution of the Coronavirus Disease 2019 (COVID-19) Blood Sample Collection under the Guideline of National Biobank Consortium of Taiwan
Investigator, Biobank Provisional Office, Department of Education and Research, Taoyuan General Hospital, Ministry of Health and Welfare, Taiwan
Dec 14, 2020
30個馬克吐溫經典英文語錄名言 (超對)
(From google Groups/sflinlab2020)
2016.11.09 15:05經典語錄網 英文佳句語錄連結
馬克·吐溫，是美國的幽默大師、小說家、作家，也是著名演說家，威廉·福克納稱讚馬克·吐溫為“第一位真正的美國作家，我們都是繼承他而來”。馬克吐溫以高超的幽默、機智與名氣，被稱為美國最知名人士之一，曾被推崇為“美國文壇鉅子”。
1、It is better to keep your mouth closed and let people think you are a fool than to open it and remove all doubt.
最好閉上嘴，讓別人認為你傻，而決不把嘴巴張開去解答所有的疑難。
2、Don’t go around saying the world owes you a living. The world owes you nothing. It was here first.
別到處說世界虧欠了你。世界什麼都不欠你的，你還沒出生它就在這兒了。
3、To succeed in life, you need two things: ignorance and confidence.
生活的成功需要兩個因素：愚昧以及自信。
4、If you pick up a starving dog and make him prosperous, he will not bite you. This is the principal difference between a dog and a man.
如果你收養了一隻饑餓的狗，並且讓它過上了舒服的日子，它將不會咬你。這便是狗與人最主要的區別。
5、Habit is habit, and not to be flung out of the window by any man, but coaxed downstairs a step at a time.
習慣就是習慣，誰也不能將其一下子扔出窗外，只能一步一步地引下樓。
6、The secret of getting ahead is getting started. The secret of getting started is breaking your complex overwhelming tasks into small manageable tasks, and then starting on the first one.
取得進展的秘訣就是開始;開始的秘訣就是將令人窒息的複雜任務，細化成可操作的任務，然後開始做第一件。
7、Kindness is the language which the deaf can hear and the blind can see.
善良是一種聾子能聽見、盲人能看見的語言。
8、The best way to cheer yourself up is to try to cheer somebody else up.
讓自己高興起來的最好辦法就是設法讓別人高興起來。
9、There are basically two types of people. People who accomplish things, and people who claim to have accomplished things.The first group is less crowded.
世界上主要有兩類人。第一類人有所成就，第二類人號稱自己有所成就。第一類人不如第二類人那麼多。
10、Every one is a moon, and has a dark side which he never shows to anybody.
人人都是月亮，都有決不讓人知道的陰暗面。
11、I was seldom able to see an opportunity until it had ceased to be one.
我總是等機會錯過了才發現我曾有過機會。
12、Wrinkles should merely indicate where smiles have been.
皺紋應該只是微笑留下的印記。
13、The human race has one really effective weapon, and that is laughter.
人類有一件真正有力的武器，那就是笑。
14、Life would be infinitely happier if we could only be born at the age of eighty and gradually approach eighteen.
如果我們能在80歲的時候出生，然後慢慢走向18歲，那生活將會幸福無比。
15、Twenty years from now you will be more disappointed by the things that you didn’t do than by the ones you did do. So throw off the bowlines. Sail away from the safe harbor. Catch the trade winds in your sails. Explore. Dream. Discover.2
20年後，讓你覺得更失望的不是你做過的事情，而是你沒有做過的事情。所以，解開帆索，從安全的港灣裡揚帆出行吧。乘著信風，去探索，去夢想，去發現!
16、Keep away from people who try to belittle your ambitions. Small people always do that, but the really great make you feel that you, too, can become great.
遠離那些企圖讓你喪失雄心的人吧。小人經常如此，而真正的偉人會讓你覺得你也可以變得偉大。
17、The only way to keep your health is to eat what you don’t want, drink what you don’t like, and do what you’d rather not.
保持身體健康的唯一辦法，就是吃點你不想吃的，喝點你不想喝的，以及做點你不願做的事情。
18、When you fish for love, bait with your heart, not your brain.
垂釣愛情用心而不是用腦做誘餌。
19、A man’s character may be learned from the adjectives which he habitually uses in conversation.
一個人的性格可以到他交談中使用的形容詞中去瞭解。
20、Get your facts first, then you can distort them as you please.
首先弄到事實，然後你就可以隨心所欲地把他們扭曲。
21、Really great people make you feel that you, too, can become great.
真正偉大的人讓你產生你也能偉大起來的感覺。
22、The more you explain it, the more I don’t understand it.
你解釋越多，我越難理解。
23、Apparently there is nothing that cannot happen today.
今天顯然什麼也不會發生。
24、The fear of death follows from the fear of life. A man who lives fully is prepared to die at any time.
先是怕生，後才怕死。完全活過的人，隨時都準備死。
25、The more things are forbidden, the more popular they become.
越是禁止的事物越流行。
26、Never argue with stupid people, they will drag you down to their level and then beat you with experience.
永遠不要跟蠢人爭論，他們會把你的層次降到同他們一樣低並用他們所謂的經驗教育你。
27、The dog is a gentleman, I hope to go to his heaven not man’s.
狗是真正的紳士，我希望去他的天堂，而不是人類的。
28、Courage is resistance to fear, mastery of fear, not absence of fear.
勇氣就是抵禦恐懼的能力，是駕馭它，而不是消滅它。
29、It’s easier to fool people than to convince them that they have been fooled.
愚弄别人比使他们相信他们已经被骗更容易。
30、Give everyday the chance to become the most beautiful day of your life.
把每天都過成你生命中最美好的一天。
Dec 7, 2020
Journal Club for Immunology
2020/12/10 (終場秀 👏👏👏)
Persistent Polyfunctional Chimeric Antigen Receptor T Cells That Target Glypican 3 Eliminate Orthotopic Hepatocellular Carcinomas in Mice
(PubMed Link) 彭佳敏
2020/11/24
Blockade of the Phagocytic Receptor MerTK on Tumor-Associated Macrophages Enhances P2X7R-Dependent STING Activation by Tumor-Derived cGAMP
(PubMed Link) 陳明玉
2020/11/12
Early precursor T cells establish and propagateT cell exhaustion in chronic infection
(PubMed Link) 謝小燕
2020/10/29
Loss of Bcl-6-Expressing T Follicular Helper Cells and Germinal Centers in COVID-19
(PubMed Link) 蔡佩汝
國家級人體生物資料庫整合平台 (NBCT)
(from groups.google.com/News Dashboard) (treasureCollection)
未來城市@天下 <-- 連結
三支利箭推升台灣的精準醫療
衛福部指出，台灣在精準醫療所射的第一支箭是99年通過《人體生物資料管理條例》，第二支箭是101年中研院成立台灣人體生物資料庫（Taiwan Biobank）。台灣人體生物資料庫依據不同遺傳氏族在臺灣地區居住分佈的特性，並針對本土常見疾病（如高血壓、糖尿病、癌症等）進行長期追蹤研究。在研究設計上採取世代研究、病例對照研究與藥物基因體研究等多重方法。世代研究將募集20萬名一般民眾及10萬名常見的10～15種疾病患者一同參與，收集參與者的健康情形、醫藥史、生活環境資訊與生物檢體、健康變化情形與治療狀況。截至108年10月底，台灣人體生物資料庫累計的參與總數超過12萬，透過這些參與總數幫助我們提升醫療品質。
集合眾人力量 Biobank共享時代來臨
目前台灣已核准成立31家人體生物資料庫，檢體總建置數高達450萬件，但受限規模大小不一，且申請程序繁複，缺乏統合，導致運用效益有限。為突破此困境，行政院政務委員吳政忠邀集中央研究院、衛福部、國衛院等部會，共同研商規劃建立「國家級人體生物資料庫整合平台」，整合國內現有人體生物資料庫，並於今年10月30日成立Biobank整合平台聯盟。現在透過單一窗口和簡便的審核程序，醫學研究人員、專家學者可以一次取得所有的資料，觀察到大量的基因，讓研究成果更準確。
人機協作 生醫產業數位加值
有關於未來生醫產業如何利用數位科技，國衛院病理核心實驗室主任黃秀芬以組織病理為例說明。過往癌症病患的腫瘤切片都是由病理科醫生用肉眼看顯微鏡來做判讀和診斷，包括良性、惡性、罹患哪一種疾病……等細節。但目前透過全玻片影像掃描等數位科技輔助，由病理醫生協助定義有哪些重要的異常判別特徵，再由程式設計師編寫在程式中，經由一系列分析衍生出數據與診斷。運用AI判讀的數位醫療，仍有醫生負責最後把關，做出正確診斷和處理。因此，人機協作輔助醫生進行診斷判讀，提升診斷速度與精準度，最大益處就是降低醫生的工作負擔。
展望未來的生醫產業人才培育，需要跨領域的多元培育。黃秀芬強調，過往僅需要連結基礎研究與臨床醫學，未來的生醫產業人才不但要懂基礎研究與臨床醫學，還要了解生物技術（Biotech）相關科技，包括基因、大數據等，才有能力與醫生對話，解決醫生在臨床上的問題。黃秀芬也以美國的就業現況為例，預告基因分析、基因判讀等領域也將成為未來就業市場的熱門專業。
行政院政委吳政忠表示，生醫科技是我國政府的5+2科技產業政策之一，我國發展生醫科技具備眾多優勢，包括行之多年的健保制度與資料庫、ICT產業創新先進、科技與醫療產業的人才資源，再加上人體生物資料庫若能做更有效的統整，將成為醫界、學界與產業界研究的最佳利基，可望進一步結合人工智慧、大數據、物聯網，實現精準醫療、智慧醫療、遠距醫療與預防醫學。
1 reply from Su-Fang: https://nbct.nhri.org.tw/
Nov 29, 2020
英試推抽血驗50種癌 全國癌症死亡人數預計大降2成
(From google Groups/sflinlab2020)
2020/11/28 13:11 (LibertyTimes)
〔即時新聞／綜合報導〕癌症年年在十大死因霸榜，讓現代人聞之色變，不過隨著醫療進步，癌症不再被視為絕症，能否及早發現便成了保命關鍵。英國國民保健署（NHS）將與加州癌篩新創企業GRAIL合作，首度實施僅靠單次驗血便可檢測50多種癌症的癌篩措施，預計將可大幅減少2成罹癌死亡人數。
根據《CNN》報導，美國加州醫療保健公司Grail近日宣布，英國國民保健署將利用該公司的Galleri血液測試技術，對16萬5000位英國民眾實施癌症檢測，受測者僅需進行單次抽血檢驗，便可針對50多種癌症進行早期篩檢，偽陽性率均低於1％。
英國國民保健署在27日發布的聲明中，以「全球首次」來形容本次癌症篩檢計畫，據稱，本次計畫將分成2階段進行，第1階段將針對13萬5000名年紀介於50至79歲無癌症跡象的民眾實施，第2階段將納入2萬5000名具有疑似癌症跡象的民眾。
英國國民保健署指出，在英國，每天有超過1000人被診斷罹癌，其中大約有一半是在第1、2期被診斷出來的，但該機構的目標是在2028年前，將早期篩出的比例增加至75％。
英國國民保健署希望血液癌篩能鎖定目前較難早期診斷及治療的癌症，「若能在早期篩出，對於像是胰腺癌及卵巢癌這類癌症特別有幫助，可以挽救許多性命」，據稱，若將Galleri血液測試技術加入該國常規護理標準，將可讓中晚期才被診斷出來的病患數量減少將近一半，並連帶導致英國癌症死亡人數大幅下降約20％
Nov 27, 2020
罹患口腔癌壽命少20年 台灣國健署：口內出現五徵兆應盡速就醫
(From google Groups/sflinlab2020)
(台灣英文新聞/生活組 綜合報導)根據衛服部死因統計和國健署癌症登記資料，台灣每天有9名死於口腔癌，21人罹患口腔癌，尤其好發於中壯年族群，罹癌後平均壽命還整整少了一般人20年。國健署王英偉署長提醒，為預防口腔癌的發生，除拒絕使用檳榔、菸品外，還要定期口腔黏膜檢查，及注意口腔黏膜變化，可有效降低口腔癌發生及死亡風險，亦可以避免後續多重癌症之發生。
根據國健署所進行的國民健康訪問調查顯示，有嚼檳榔者7成有吸菸習慣，6成6長時間有喝酒，檳榔、菸、酒等習慣皆有者近5成。過去研究指出嚼檳榔造成口腔癌的機率為未嚼者的28倍。
嚼檳榔不僅會增加口腔癌發生機率，也會對上呼吸消化道(口腔、咽、喉、食道)造成傷害，近年研究指出嚼檳榔者罹患上呼吸消化道癌症風險，較不嚼檳榔者之風險高5倍，若檳榔、菸、酒三者皆有使用習慣，較無使用者罹患上呼吸消化道之風險更高達10.5倍!
國健署提醒「拒絕檳榔、定期檢查、及早就醫」才是預防口腔癌不二法門。早期發現口腔病灶是提升存活率與降低治療難度的關鍵，民眾若發現口內出現「破斑硬突腫」之一的情形，應盡速就醫：
破：口腔黏膜破皮超過兩週未癒合
斑：口腔黏膜出現不明的紅斑、白斑
硬：口腔黏膜逐漸變硬、緊，最終嘴巴張不開
突：口腔黏膜表面不規則突起(乳突狀或疣狀突起)
腫：臉或頸部出現腫塊或兩側不對稱
口腔癌是最容易預防的癌症！國民健康署呼籲國人要拒絕檳菸酒，定期篩檢、及早戒除。口腔黏膜檢查的目的，是要找出癌前病變，加以治療阻斷癌症發生的機會，提醒30歲以上有菸檳行為的民眾，每2年攜帶健保卡至健保特約牙科、耳鼻喉科醫療院所接受口腔黏膜檢查，若自己發現有「破、斑、硬、突、腫」其中一項症狀，要盡速就醫接受治療，就可預防口腔癌!
Nov 12, 2020
打擊假訊息 外交政策：西方國家應向台灣學習
(from groups.google.com/News Dashboard) (treasureCollection)
中央社記者江今葉華盛頓11日專電）「外交政策」刊登專文指出，全球飽受假訊息之苦，但台灣打擊假訊息的成功經驗顯示，最好的防禦來自進攻。西方國家應向台灣這樣的創新者學習，贏得這場不對稱的反民主戰爭。
這篇由前美國外交官柯爾（Walter Kerr）與曾在前美國總統歐巴馬（Barack Obama）任內擔任白宮新媒體主管的菲力普斯（Macon Phillips）今天在「外交政策」（Foreign Policy）共同發表的文章，以去年12月總統大選前台灣充斥假訊息為例指出，儘管許多荒誕的假訊息流傳，總統蔡英文仍獲得壓倒性勝利，「假新聞失敗」。
在臉書（Facebook）與YouTube流傳販賣兒童、疫苗接種風險等陰謀論之際，台灣讓所有人對生活在正常新聞環境仍存有希望。若是西方國家對虛假訊息的反應能遵循台灣模式並採取積極立場，西方應能戰勝假訊息。
文章指出，台灣採取全社會途徑，透過公民技術社群與社群媒體合作，針對可疑訊息隨時進行分析確定其正確性，獨立的台灣事實查核中心也設立事實查核網路資料庫。 (原文連結)
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