Feb 19, 2012

0215Lab meeting-蔡小丸

1. 完成三組tissue microarray的DDR1, collagen type 1及collagen type 4的染色, 初步計分結果, 似乎DDR1的大量表達與collagen type1的大量表達較為相關,而DDR1大量表達也與疾病的grade較相關
2. 比較OSCC cells DDR1 tyrosine phosphorylation的情形, 結果為DOK, C9, OC-3, OEC-M1, TW2.6>HSC-3, SCC-15, T47D>HEK293, 發現DDR1 tyrosine phosphorylation的程度與gilvec對於這些細胞的抑制能力,沒有很好的相關性, 然而發現在OEC-M1, TW2.6, HSC-3, SCC-15這幾組IP DDR1 sample中, 發現約略80-85kDa的DDR1-associated protein有強烈的tyrosine phosphorylation, 但此蛋白為何未知
3.此外 DDR1的活性反映出的下游為何未知 (但排除非p-MAPK及p-AKT)
4.利用Q-PCR分析一系列oral cells的DDR1 isoforms的表達, 結果發現大多細胞只表達1a及1b這兩種isoforms, 而mRNA表達量也與蛋白表達量具相關性
張貼者： 於

2 comments:

  1. SFL2/19/12, 10:19 PM

    Good! We also should strive for Mass Spect and Micro-Western.

    ReplyDelete
  2. SFL2/19/12, 10:21 PM

    The other two things are requesting plasmids from Prof Tang and asking Dr. Chu PY for the helps on TMA results.

    ReplyDelete

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